- "Loki means to unleash the Hulk."
- ―Black Widow
Hawkeye, with the permission of Nick Fury, tested the security of the Helicarrier in a faux hostile insertion. He was able to infiltrate the ship and get through many of its checkpoints. He advised Fury of the ship's flaws that he was able to exploit.
In the Destruction of Project P.E.G.A.S.U.S., Loki had returned to Earth to conquer it. After destroying the Joint Dark Energy Mission Facility and stealing the Tesseract, Loki traveled to Stuttgart, Germany to steal Iridium. Captain America and Black Widow traveled to Stuttgart in a Quinjet to confront him. Loki gets the upper hand on Rogers until Iron Man arrives and Loki surrenders. They take Loki into S.H.I.E.L.D. custody aboard the Helicarrier, where he was placed in a confinement capsule designed to hold the Hulk. Loki taunted Nick Fury about his attempts to control the Tesseract and his gathering of misfits to defend Earth.
Loki's mere presence was enough to provide a disruptive element that would fragment the only group of heroes that had any hope of stopping him.
- "Your heroes are scattered; your floating fortress falls from the sky. Where is my disadvantage?"
When Black Widow arrived to speak with Loki, apparently to offer a deal in exchange for Hawkeye's freedom, he subjected her to a play of wits where he ferreted out her deeper misgivings, but was completely caught off guard when the spy tricked the trickster into revealing that there was already a monster among them and that it was not him. From this, she concluded that the monster was Bruce Banner and that he had planned to use the Hulk to destroy the ship and the heroes as well.
However Loki's plan to use the Hulk, only one aspect of his real intentions, did follow through as another Quinjet carrying Barton and the rogue agents came to his aide, following a signal from the Scepter. Barton uses an explosive arrow to destroy one of the rotors and lands on the Helicarrier. The explosion knocks Romanoff and Banner to a lower lever and the wounded Banner transformed into the Hulk. Banner chases Romanoff until Thor intervenes and begins fighting Banner.
Barton and the brainwashed agents head to the bridge and Nick Fury and Maria Hill kill them but Barton uses a special arrow to hack the computers and disable another rotor. Fury orders an F-35 Lightning II to fire on Banner, who jumps on the jet and tears it apart. The pilot ejects and Banner falls to the ground.
While everyone else was fighting the attackers, Iron Man and Captain America fixed the rotor that the attackers destroyed. After getting the rotor moving, Stark got trapped inside until Rogers defeated two of the brainwashed S.H.I.E.L.D. commandos and pulled the lever to slow the rotor. Romanoff intercepts Barton on his way back to the Quinjet. The two fight for a while until Romanoff hits Barton in the head hard enough to break his brainwashing.
Loki then used illusions of himself to trick Thor into entering the cell. Agent Phil Coulson arrives but Loki uses another illusion to fool him and stabs Coulson in the back with the Scepter and ejected Thor's cell from the Helicarrier. Before Loki can leave Coulson shoots him with the Destroyer Armor Prototype Gun. Loki escapes on the Quinjet and Fury and the medics get to Coulson but it is too late.
- "I'm clearance Level 6. I know that Agent Coulson was killed in action before the battle of New York. Got the full report."
"Welcome to Level 7."
- ―Grant Ward and Phil Coulson