Ming-Na Wen portrays Melinda May in Agents of S.H.I.E.L.D.
She also portrayed Agent 33 in disguise in Face My Enemy, Ye Who Enter Here, What They Become, Love in the Time of HYDRA, and S.O.S. Part Two.
She portrayed herself in Agents of S.H.I.E.L.D.: Double Agent and Agents of S.H.I.E.L.D.: Academy.
She was interviewed for the documentaries Marvel Studios: Assembling a Universe, Marvel 75 Years: From Pulp to Pop and Captain America: 75 Heroic Years.
- She was nominated for a People's Choice Award for Favorite Actress in a New TV Series for her work on Agents of S.H.I.E.L.D.
- She was nominated for a Kids' Choice Award for Favorite Female TV Star - Family Show for her work on Agents of S.H.I.E.L.D..