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As Phil Coulson, Melinda May and Lincoln Campbell were preparing to after Hive, Coulson said to Campbell that if would go with them, he had to wear the Nanothermite Vest. Campbell disapproved of wearing, but eventually he agreed and wore it during the investigation of Alisha Whitley.
Campbell and Yo-Yo Rodriguez wore the Nanothermite Vest during the Battle of the Missile Silo. During the mission, Rodriguez asked Alphonso Mackenzie if he would let her explode if she would be swayed by Hive. She told him she rather die than turning into someone's slave.