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Parker accidentally activated a new function in his Web-Shooters while checking them when his aunt May Parker entered into his room to take care of his blackened eye, forcing Parker to hide the device behind a cushion. Once she left, he noticed a red light beam being projected from the Web-Shooter and looked at the roof to notice a signal very similar to the mask of his new costume. Parker watched the signal in delighted surprise.
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- In the comics, the Spider-Signal is usually emitted from Spider-Man's belt instead of his Web-Shooters.