- Stan Lee made a guest star appearance in I'm a Furious Yellow and Married to the Blob.
- Homer got dipped in green paint and got angry and looked like the Incredible Hulk while Stan Lee trys to turn into the Incredible Hulk himself.
- Homer, Lenny, Carl and Barney dressed as the Fantastic Four.
- The Simpsons in a Treehouse of Horror episode turned into the Fantastic Four
- Stan Lee jammed a Thing action figure into a boy's Batmobile.
- Comic Book Guy dressed up as the Wolverine to take a flew shot so he would be less afraid of the needle.
- A figurine called the Badgerine in Comic Book Guys shop is an obvious parody of the Wolverine.
- Doctor Hibbert was wearing a Captain America costume.
- Lois dressed up as Mystique in the episode "8 Simple Ways for Buying My Teenage Daughter"
- Peter dressed up as Spider-Man in the episode "And Bango Was His Name-Oh".
- Spider-Man himself made appearances on the show.
- Peter turns into the Incredible Hulk in a cutaway gag in the episode "Chitty Chitty Death Bang".
- Iceman made an appearance in a cutaway gag in the episode "Hell Comes to Quahog".
- The Thing made an appearance in a cutaway gag in the episode "Bill and Peter's Bogus Journey".
- Iron Man was seen as a Iron Chef in Peter's rehearsal for a cutaway gag in the episode "3 Acts of God".
- In "Something Something Dark Side" Yoda had Iron Man on DVD.
- Barry was wearing a Spider-Man shirt in the episode "With Friends Like Steve's"
The Cleveland Show
- Cleveland Brown Jr. made a robotic armor out of kitchen appliances and in the form of Iron Man.
Malcolm in the Middle
- Hal was couching a children's soccer team and was inspiring them by being like the X-Men.
- The boys dress up as the Avengers for Halloween.
- In a Thanksgivings episode the plot of the pilgrims and native americans the episode parodies the film Thor.
- Howard the Duck is mentioned by ALF.
- Spider-Man and his origin's are mentioned in an episode of CSI.
- The son of Criminal Minds character Aaron Hotchner is a fan of Captain America.
- Dexter is a fan of heroes Major Glory and Klunk, parodies of Captain America and the Hulk, respectively. It later goes from reference to full-on parody when these heroes appear in the "Dial M for Monkey" segments (which already included a SHIELD pastiche) and then they get their own segments as the "Justice Friends".
- When Dexter tries to get super-powers, one of the methods he uses is to be bitten by a radioactive spider like Spider-Man.
The Fairly Odd Parents
- At the beginning of "Abra-Catastrophe!", Timmy pretends that he is Spider-Man, with Cosmo as the Green Goblin.
Teenage Mutant Ninja Turtles
- Michelangelo's last rival in the Battle Nexus tournament, who can turn into a muscular form to fight, is named Kluh and comes from planet Levram, which are Hulk and Marvel respectively backwards. Kluh's father is named Ammag (Gamma backwards).
- The Marvel comic Spidey Super Stories tied in with The Electric Company and Spider-Man's appearances on the show. Issue #31, "Star Jaws," featured a Star Wars parody in which Sam the Robot made a special appearance, billed as "Sam, the Sesame Street Robot." Filling the roles of both R2-D2 and C-3PO, Sam sought Spider-Man's assistance in an intergalactic war.
- Spider-Man guest starred in the Muppet Babies episode "Comic Capers," produced by Marvel Productions (whose logo, seen at the end of each Muppet Babies episode, also featured a CGI Spider-Man). Baby Rowlf and Baby Skeeter entered the Spider-Man newspaper comic, and attempted to foil bank robbers. They only succeeded in irritating Stan Lee, in a live-action cameo, who pointed out there was only one Spider-Man. The closing gag featured Baby Animal and Baby Gonzo disguised as Spider-Man, with Animal on top wearing the mask, until they collide with a building and Gonzo falls from the bottom of the costume.
- Spider-Man is pictured on the cover of the tenth issue of the Muppet Babies comic book, along with many other Marvel superheroes, as part of the celebration of the company's 25th anniversary.
- At the end of a 1974 episode of Sesame Street, Big Bird, caught in a series of string, remarks that he feels like Spider-Man.
- The 1975 book See No Evil, Hear No Evil, Smell No Evil depicts an Electric Company poster featuring "Spidey".
- In Tales of a Sixth-Grade Muppet, an illustration of a Spider-Man comic book is seen, and Danvers' sister Chloe once posted a video of him wearing Spider-Man underwear.
- A parody sketch from Sesame Street Episode 4273 features a spoof of "Spider-Man: Turn Off the Dark", where Grover portrays "Spider-Monster" and keeps falling on Mr. Johnson. Bono (a spoof of the play's co-composer) appears as a stage hand.
- In Tales of a Sixth-Grade Muppet: The Good, the Bad, and the Fuzzy, when Danvers speculates that becoming a superhero must be the reason for his Muppet transformation, he notes "It's like when Spider-Man got bit by that radioactive spider!"
- The Incredible Hulk appeared in the 1989 Muppet Babies episode "Comic Capers", using original footage from Marvel Productions' 1985 animated series, in the song "The Sunday Funnies." He later mades a cameo appearance when Baby Rowlf (portrayed as Peter Barker) opened up an elevator door (with the growls voiced by Frank Welker.)
- A shot of a child wearing an Incredible Hulk sweatshirt was shown in the "Global Grover" segment on Sesame Street about Angola.
- Kermit tells the publishers that they wouldn't like Miss Piggy when she's angry. Walter gives out a chuckles and points out the Hulk reference in a web video for Entertainment Weekly.
- Danvers told the reader in Tales of a Sixth-Grade Muppet: Clash of the Class Clowns, "In case you haven't heard, I have to share bunk beds with my sister, which is only slightly less embarrassing than having your mom walk in on you when you're covered in green Magic Marker and reciting lines out loud from The Incredible Hulk (don't ask me how I know this)."
- Danvers told the reader in Tales of a Sixth-Grade Muppet: The Good, the Bad and the Fuzzy that his arch-nemesis Beebus Spracklin had been known as the Incredible Hulk of their elementary school.
- Cookie Monster played the Edible Hulk in a parody of The Avengers in an Entertainment Weekly video.
- Scooter's bus locker from the book and record version of The Muppets Takes Manhattan in a photo includes personalized sports banners and an issue of Ghost Rider.
- Kermit interviewed Telly and Mona at a daycare center in a Sesame Street News Flash, standing in front of pictures of Marvel heroes Thor and Captain America.
- The X-Men movie franchise was parodied in Statler and Waldorf: From the Balcony episode 24 featuring a group of mutant auditions, including The Blimp, The Invisible Twins and Beaverine.
- The Muppets can be seen wearing Captain America and Thor costumes in a variant cover for The Muppet Show Comic Book: Family Reunion #7.
- Grover became "Iron Monster", a parody of Iron Man, in Sesame Street Episode 4216.
- Telly Monster, Abby Cadabby and Elmo pretended to become the "Furry Four", a spoof of the Fantastic Four, in Sesame Street Episode 4218.
- Sam the Eagle appeared as Captain America in a 2011 Muppets parody poster.
- Walter wore a Captain America T-shirt and kept wondering if the publishers had an advanced screening of The Avengers in a web video with Kermit and Miss Piggy for Entertainment Weekly.
- Grover and Cookie Monster played Super Grover and the Edible Hulk in a spoof of The Avengers in a web video for Entertainment Weekly.
Off the Mark
- A few Marvel characters, including Spider-Man, The Thing, The Human Torch, The Incredible Hulk and Iron-Man appeared in a few Sunday strips for The Betty Rubble Center: For Recovering Toons, usually taken around the last sunday of Monday every year since 2001 (different dates from 1996-2001 varied).
- Issue #25 features the Ax, a secret organization with a massive airship, akin to SHIELD and the Helicarrier.