During the HYDRA Uprising, Preston Case, alongside with Alphonso Mackenzie, Susanna Lauren Sherman, and Tim Maguire were captured by HYDRA. He witnessed how the leader Dean Haddad of the assault killed Maguire. He and the two others were saved by Bobbi Morse and Isabelle Hartley who managed to kill the HYDRA agents.
The five agents searched for Robert Gonzales and after they found him, Preston helped him to recover from his broken leg; Morse told him that he did well though he was a trainee. Morse then revealed that she was given a secret mission by Nick Fury to destroy the ship before HYDRA will get their hands on his cargo. Gonzales tried to persuade her to continue the mission but she listened to Mackenzie instead, who said to her to ignore Fury's order and claim back the ship. Despite not been a skilled fighter, Case fought with the other agents against the rest of HYDRA on the ship and helped to reclaim it.
- S.H.I.E.L.D. (First Incarnation)