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The Vita-Ray Chamber is a specially constructed medical chamber that harnesses and projects beams of concentrated Vita Radiation. The chamber also contains several mechanisms used for injecting subjects with the Super Soldier Serum.
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The Vita-Ray Chamber was a key component of the process used for Project Rebirth, the 1940s experiment that transformed Steve Rogers into Captain America. The chamber was created by the Strategic Scientific Reserve to administer the Vita Radiation developed by Dr. Abraham Erskine. The chamber was constructed, in part, and operated by Howard Stark. During the experiment, the chamber required a tremendous amount of energy and was hooked directly to the power grid of 40s era New York City. The procedure was a success, transforming the formerly sickly Steve Rogers into a muscle bound super soldier.
- In the Hulk and the Agents of S.M.A.S.H. episode Days of Future Smash: Year of the Hydra the Hulk follows Leader back in time to World War II where the Red Skull recreates the Vita-Ray Chamber. With the Leader's help, the Red Skull uses the Gamma Radiation instead of the Vita Radiation to transform himself into the Hulk-sized Green Skull.